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M4 version-2 held-out benchmark proposal

Status: proposal, 2026-10-17. Not a lock; nothing here is scored.

Scope: M4 (#36), S3. This proposes eligibility for a later manifest, not a new experimental result.

Why a v2 is needed

The v1 manifest has no unused scored case left. Its only scored held-out case, ORN_DM1 → DM1_lPN, ran and failed specificity and stability. ORN_V is reported only and does not decide. No exploratory row in the benchmark inventory is promoted. A new source must not have selected the model. The completion plan requires a pass against untouched animal observations; a no-go does not close M4.

Eligibility rules

All must hold, with the rules written before any candidate response data are opened:

Candidates assessed

These are feasibility findings from the supplied entry-protocol search and type match analysis, without opening response files. No candidate is eligible now.

Source Evidence and limits Verdict
Baker et al. 2022 24 new WED/VLP types plus A2, B1, B2 and WED-VLP: 28 consistently responding classes. Princeton deposit 10.34770/7zem-x425: CC BY-NC-ND 4.0, 3 files, about 2.3 MB; not opened. FlyWire route detailed below. Virgin females; most matched types have no dimorphism annotation. Eligible after protocol 6 input, identity completion where needed, and sex evidence, under the licence scope below; AVLP_pr36 excluded.
Deutsch et al. 2019 Dsx types pC1, pC2l, pC2m, pMN1 and pMN2; mostly dimorphic. Female auditory pC1 has no corresponding male response; pMN2 is female-specific. No resolved cited class-to-MaleCNS mapping; raw data on request. Not eligible now.
Matsuo 2014; Ishikawa 2017; Patella & Wilson 2018 JO calcium/driver-line measurements; female or unresolved type correspondence; figures only, no deposit or on request. Not protocol 6's input. Not eligible.
Yang 2016; Pang 2025 No measured paired lamina → medulla gain; Pang has no medulla readout. Not eligible.
Kazama & Wilson 2009; Augustin 2019; Kohn 2021; Strother 2018; Apostolopoulou & Lin 2020 Missing conductance/input resistance, fitted conductance, unavailable per-type traces, calcium without gain, or unresolved APL baseline/dynamic range. Not eligible.
Dombrovski 2023/2025; Klapoetke 2017; Jang 2023; Zhao 2025; DANDI 001869 No identified LC4/LPLC2 spike input. Not eligible.
Hengstenberg 1992; Ispizua 2026; Lobato-Rios 2022 Tethered/undefined angle reference, unavailable keypoints/licence, or one fixed female; no qualified resting registration. Not eligible.
FlyGym 2.1.0; Fenwick 2026; Azevedo 2020 Joint naming, chemically activated myofibrils, or tibia rather than TTM; no required muscle → actuator chain. Not eligible.

Baker's route is Table S3 root IDs → flywire_annotations v3.2.0, materialization 783 → exact MaleCNS flywireType tokens. Annotation sources: Schlegel et al. 2024 and Berg et al. 2026. The CAVE coordinate/lineage audit extends complete named-type coverage from 2 to 12 of 28 classes: 11 retain unresolved sex compatibility and AVLP_pr36 remains excluded for dimorphism. Fourteen classes are partial and two (AVLP_pr18 and AVLP_pr24) are absent from Table S3. All 404 coordinate anchors agree with both source root-ID lineages at materialization 783; 332 reconstructions have named MaleCNS routes. Complete coverage may be one-to-many, not a single modern type. The older name/synonym search does not override the cited route. No physiological response data were opened and no class is benchmark eligible.

Baker licence basis and publication scope

For the proposed noncommercial scientific benchmark, CC BY-NC-ND 4.0 permits local analysis, including private transformations. ND is not an automatic bar to computing benchmark metrics. This is a scoped licence basis, not permission for commercial use or unrestricted publication of derived data.

Metadata checked on 2026-10-17, without downloading or opening response files:

The intended workflow is:

Within this scope, no separate permission is needed merely because the local analysis transforms data. Output review remains a publication gate. This does not make Baker scientifically eligible or authorize opening held-out responses before the prospective lock.

Blockers and owner decisions

Next, if unblocked

Write the calibration/exploratory/held-out partition first. Lock validation/benchmarks.yaml version 2 with a new content hash and the complete prospective rules before opening responses. Preserve v1's failure and reported roles. Then open the eligible data once under that lock; archive provenance, controls and all outcomes. Baker, Deutsch and Pacheco response data remain unopened here.